![]() ![]() albida et le développement de son protocorme. L’auteur a expérimenté un large spectre de milieux de culture conçus pour d’autres orchidées terrestres, afin d’identifier des composantes cruciales pour la germination des graines matures du P. Ce dernier groupe inclut le Pseudorchis albida, une orchidée terrestre verte dont les populations connaissent un déclin considérable sur l’ensemble de son aire de distribution. ![]() Bien que certaines orchidées germent facilement en conditions aseptiques in vitro, plusieurs demeurent récalcitrantes sans raison apparente. Les orchidaceae constituent une des familles de plantes les plus menacées et les techniques de propagation in vitro jouent un rôle irremplaçable pour sa conservation. ![]() albida may inhibit its recruitment and might therefore represent one of the major causes of the decline of P. Our results imply that even very low nitrogen depositions in natural populations of P. The highest germination rates were obtained on media containing both kinetin and sucrose and no nitrates, while the fastest protocorm development occurred on media lacking kinetin. Trehalose inhibited seed germination but slightly promoted protocorm development. Nitrates strongly inhibited both germination and protocorm development, even at surprisingly low concentrations (2 mg NO 3 − ![]() Specifically, we focused on nitrate concentrations and the presence or absence of trehalose, sucrose, and kinetin. albida mature seed germination and protocorm development. We tested a wide spectrum of cultivation media designed for other terrestrial orchids to identify key components for P. The latter group includes Pseudorchis albida, a green terrestrial orchid whose populations are in considerable decline throughout its distribution range. While some orchids germinate readily in asymbiotic conditions in vitro, many are reluctant for no obvious reason. Orchidaceae is one of the most endangered plant families, and in vitro propagation techniques play an irreplaceable role in its conservation. ![]()
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